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non immune sheep igg  (Cedarlane)


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    Cedarlane non immune sheep igg
    Non Immune Sheep Igg, supplied by Cedarlane, used in various techniques. Bioz Stars score: 85/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/non+immune+sheep+igg/Anti-Sheep+IgG+(H%2BL)%2C+IgG+Fraction%2C+(Polyclonal)+(rabbit+IgG)/us09694103-532-0-6
    Average 85 stars, based on 15 article reviews
    non immune sheep igg - by Bioz Stars, 2026-09
    85/100 stars

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    Related Articles

    Incubation:

    Article Title: Physiological changes in membrane-expressed platelet factor 4: implications in heparin-induced thrombocytopenia.
    Article Snippet: Contents lists available at ScienceDirect Thrombosis Research j ourna l homepage: www.e lsev ie r.com/ locate / th romres Regular Article Physiological changes in membrane-expressed platelet factor 4: Implications in heparin-induced thrombocytopenia☆ M. Margaret Prechel ⁎, Walter P. Jeske, Jeanine M. Walenga

    Affinity Purification:

    Article Title: Physiological changes in membrane-expressed platelet factor 4: implications in heparin-induced thrombocytopenia.
    Article Snippet: Contents lists available at ScienceDirect Thrombosis Research j ourna l homepage: www.e lsev ie r.com/ locate / th romres Regular Article Physiological changes in membrane-expressed platelet factor 4: Implications in heparin-induced thrombocytopenia☆ M. Margaret Prechel ⁎, Walter P. Jeske, Jeanine M. Walenga

    Purification:

    Article Title: Physiological changes in membrane-expressed platelet factor 4: implications in heparin-induced thrombocytopenia.
    Article Snippet: Contents lists available at ScienceDirect Thrombosis Research j ourna l homepage: www.e lsev ie r.com/ locate / th romres Regular Article Physiological changes in membrane-expressed platelet factor 4: Implications in heparin-induced thrombocytopenia☆ M. Margaret Prechel ⁎, Walter P. Jeske, Jeanine M. Walenga



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    Image Search Results


    ( a ) Western immunoblotting analysis of phosphorylation of PDGFRβ of vehicle-, PDGF-BB- and IL-33-treated lung pericytes cultured in vitro . Beta-actin indicates loading levels. ( b ) Western immunoblotting analysis of Erk phosphorylation, Akt phosphorylation of vehicle-, PDGF-BB- and IL-33-treated pericytes. Beta-actin indicates loading levels. ( c ) qPCR analysis of Il33 mRNA of vehicle-, U0126- or Akti-1/2-treated PDGF-BB-stimulated or non-stimulated lung pericytes cultured in vitro . (PC; n =6 samples per group). NS, not significant. ( d ) Heatmap profiling of transcription factor gene expression of vehicle- and PDGF-BB-stimulated lung pericytes cultured in vitro . ( e ) qPCR analysis of Sox7 mRNA expression levels of anti-PDGFRα- or anti-PDGFRβ-treated lung pericytes that received PDGF-BB-stimulation ( n =6 samples per group). Vehicle-treated pericytes served as controls ( n =6 samples per group). NS, not significant; PC, pericyte. ( f ) qPCR analysis of Il33 mRNA expression levels of vehicle- or PDGF-BB-stimulated lung pericytes that were transfected with scrambled or Sox7 siRNA ( n =6 samples per group). PC, pericyte. ( g ) Schematic diagram of IL-33 expression in pericytes regulated by the PDGF-BB-PDGFRβ signalling through SOX7 transcriptional regulation. PDGF-BB-activated PDGFRβ induces SOX7 that targets the SRY boxes located in the Il33 promoter. ( h ) ChIP assay of SOX7 binding to the Il33 gene promoter. Non-immune IgG and Il33 coding region served as controls ( n =6 samples per group) (mean±s.e.m., NS, not significant, Student's t -test). Full-gel images for a , b are shown in .

    Journal: Nature Communications

    Article Title: The PDGF-BB-SOX7 axis-modulated IL-33 in pericytes and stromal cells promotes metastasis through tumour-associated macrophages

    doi: 10.1038/ncomms11385

    Figure Lengend Snippet: ( a ) Western immunoblotting analysis of phosphorylation of PDGFRβ of vehicle-, PDGF-BB- and IL-33-treated lung pericytes cultured in vitro . Beta-actin indicates loading levels. ( b ) Western immunoblotting analysis of Erk phosphorylation, Akt phosphorylation of vehicle-, PDGF-BB- and IL-33-treated pericytes. Beta-actin indicates loading levels. ( c ) qPCR analysis of Il33 mRNA of vehicle-, U0126- or Akti-1/2-treated PDGF-BB-stimulated or non-stimulated lung pericytes cultured in vitro . (PC; n =6 samples per group). NS, not significant. ( d ) Heatmap profiling of transcription factor gene expression of vehicle- and PDGF-BB-stimulated lung pericytes cultured in vitro . ( e ) qPCR analysis of Sox7 mRNA expression levels of anti-PDGFRα- or anti-PDGFRβ-treated lung pericytes that received PDGF-BB-stimulation ( n =6 samples per group). Vehicle-treated pericytes served as controls ( n =6 samples per group). NS, not significant; PC, pericyte. ( f ) qPCR analysis of Il33 mRNA expression levels of vehicle- or PDGF-BB-stimulated lung pericytes that were transfected with scrambled or Sox7 siRNA ( n =6 samples per group). PC, pericyte. ( g ) Schematic diagram of IL-33 expression in pericytes regulated by the PDGF-BB-PDGFRβ signalling through SOX7 transcriptional regulation. PDGF-BB-activated PDGFRβ induces SOX7 that targets the SRY boxes located in the Il33 promoter. ( h ) ChIP assay of SOX7 binding to the Il33 gene promoter. Non-immune IgG and Il33 coding region served as controls ( n =6 samples per group) (mean±s.e.m., NS, not significant, Student's t -test). Full-gel images for a , b are shown in .

    Article Snippet: In brief, the cells were fixed with 4% paraformaldehyde (PFA) before sonication with an agarose-blocking buffer, followed by incubation overnight with 2.5 μg of a non-immune sheep IgG (Cat. No. 12-515, Millipore) or an anti-SOX7 antibody (kindly provided by Dr Valerie Kouskoff, Cancer Research UK Manchester Institute, United Kingdom) per immunoprecipitation reaction.

    Techniques: Western Blot, Phospho-proteomics, Cell Culture, In Vitro, Gene Expression, Expressing, Transfection, Binding Assay